There are five major steps in the IVF and embryo transfer sequence: 1. Monitor the development of ripening egg(s) in the ovaries. 2. Collection of Eggs. 3. Obtaining the sperm. 4. Putting the eggs and sperm together in the laboratory, and providing optional conditions for fertilization and early embryo growth. 5. Transferring the embryos into the uterus. To control the timing of egg ripening and to increase the chance of collecting the substantial number of eggs, fertility drugs are prescribed according to each individual case. Before determining the egg retrieval schedule, we perform an ultrasound of the ovaries to check the development of eggs and a blood/urine test to measure hormone levels The Egg Retrieval and embryo transfer procedure: The retrieval procedure to eggs is performed transvaginally using a hollow needle guided by the ultrasound image (this is completely comfortable under adequate sedation and general anesthesia. An egg is gently removed from ovaries using the needle. This is called “follicular aspiration.” Our embryologist which are IVF specialist in Pune laboratories immediately identifies the eggs. They are placed with the sperm in incubators to allow fertilization to take place. the eggs are examined carefully at intervals to ensure that fertilization and cell division have taken; the fertilized eggs are now called embryos. Embryos are usually placed in the wife’s uterus 2 or 3 days after egg retrieval.